Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Yangjing capsule attenuates cyclophosphamide-induced deficiency of testicular microcirculation in mice

Baofang Jin¹ , Weihang Dong², Dalin Sun¹, Bin Cai¹, Weimin Deng¹, Yugui Cui³, Yihan Jin⁴, Li Tong², Ping Wu²

¹Andrology Department of Integrative Medicine, School of Medicine, Zhongda Hospital Southeast University, Nanjing 210009; ²Medical College of Qinghai University, XiÃ¢â‚¬â„¢ning 810001, Qinghai; ³State Key Laboratory of Reproductive Medicine, Clinical Center of Reproductive Medicine, First Affiliated Hospital with Nanjing Medical University; ⁴Center of Reproductive Medicine, School of Medicine, Zhongda Hospital Southeast University, Nanjing 210009, Jiangsu, Chin.

For correspondence:- Baofang Jin Email: hexiking@126.com

Accepted: 28 February 2020 Published: 31 March 2020

Citation: Jin B, Dong W, Sun D, Cai B, Deng W, Cui Y, et al. Yangjing capsule attenuates cyclophosphamide-induced deficiency of testicular microcirculation in mice. Trop J Pharm Res 2020; 19(3):603-608 doi: 10.4314/tjpr.v19i3.21

© 2020 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To explore the protective effects of Yangjing capsule (YC) on testicular microcirculation in a mouse model of deficiency of testicular microcirculation.

Methods: Immunohistochemistry was applied to determine the effects of YC on microvascular density of mice. The protein level of CD34 and vascular endothelial growth factor A (VEGF A) was measured by western blot. The viability of Testicular cell line (TM4 cells) was examined by CCK-8 assay.

Results: Histopathological changes demonstrated that CP-induced decrease of microvascular density of the mice was rescued by YC dose-dependently (p < 0.5). Western blot data showed that the protein levels of CD34 and VEGF A in CP group were significantly decreased, but dose-dependently increased by YC, respectively, following co-administration of CP + YC, compared with those in CP group (p < 0.5). The results from CCK-8 assay showed that the cell viability of TM4 cells increased with the amount of YC administered, and that high concentrations of YC (0.1 and 1 mg/mL) showed significant effects (p < 0.5). Moreover, YC showed little effect on VEGF A mRNA and protein expression in TM4 cells.

Conclusion: YC may be considered an alternative therapeutic agent for the management of testicular microcirculation disease. However, further studies are required to ascertain this.

Keywords: Yangjing Capsule, Testicular microcirculation, Cyclophosphamide, Vascular endothelial growth factor A